NESS? Protease (formula 4), Protease with Calcium (formula 416) and Protease with Cal-Mag (formula 419) contain protease to help boost the body’s natural healing mechanisms and combat inflammatory conditions. Formula 4 is a protease-only product. Protease with Calcium (formula 416) also contains calcium, a mineral essential to several bodily processes, including muscle contraction, nerve transmission and bone formation. Calcium also helps to decrease the rate of bone loss seen with osteoporosis. Protease with Cal-Mag (formula 419) contains both calcium and magnesium. Magnesium is also vital to bone formation, and these two minerals work together closely to regulate muscle tone. Any of the formulas above can be used alone or in conjunction when higher levels of protease are needed.